RNA Seq of Nicotiana attenuata MAGIC RIL: M1_196 - SRA

SRX18723924: RNA Seq of Nicotiana attenuata MAGIC RIL: M1_196
1 ILLUMINA (Illumina NovaSeq 6000) run: 32.6M spots, 9.8G bases, 3Gb downloads

Design: Frozen leaf tissue aliquot was shipped to NOVOGENE (HK) COMPANY LIMITED for RNA-Seq. mRNA was enriched using oligo(dT) beads and for long-non-coding libraries, rRNA was removed using the Ribo-Zero kit that leaves the mRNA. First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina), dNTPs(dUTP, dATP, dGTP and dCTP), RNase H and DNA polymerase I were added to initiate the second-strand synthesis. Then followed by purification by AMPure XP beads, terminal repair, polyadenylation, sequencing adapter ligation, size selection and degradation of second-strand U-contained cDNA by the USER enzyme. The strand-specific cDNA library was generated after the final PCR enrichment and was sequenced on Illumina Novaseq.

Submitted by: Max Planck Institute for Chemical Ecology

Study: Nicotiana attenuata AZ-2019 MAGIC eQTL

PRJNA911202 • SRP413372 • All experiments • All runs

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Whole transcriptome sequencing of OS elicited 350 MAGIC RILs including the parental lines of Nicotiana attenuata. Plants were elicited with Manduca Sexta OS and harvested after 1h.

Sample: R955

SAMN32261476 • SRS16165189 • All experiments • All runs

Organism: Nicotiana attenuata

Library:

Name: M1_196

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM PCR

Layout: PAIRED

Runs: 1 run, 32.6M spots, 9.8G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR22763243	32,571,284	9.8G	3Gb	2023-08-22

ID:: 25803520

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